Journal: Scientific Reports

Article Title: ROCK1/MLC2 inhibition induces decay of viral mRNA in BPXV infected cells

doi: 10.1038/s41598-022-21610-9

Figure Lengend Snippet: ROCK1 inhibition impairs BPXV-induced cell contraction and viral protein synthesis. ( a ) Effect of Thiazovivin on MLC2 phosphorylation (activation). Vero cells were either mock-infected or infected with BPXV at MOI of 5. Thiazovivin or vehicle controls were added at 4 hpi. Cell lysates were prepared at 9 hpi and subjected for detection of the p-MLC2 levels in Western blot analysis (ai). The histogram (aii) shows the band intensity of the protein. The blots were quantified by densitometry (ImageJ) and the data are presented as mean with SD. n = 3 independent experiments. See Supplementary Fig. for full blots. ( b ) Effect of Thiazovivin on BPXV induced cell contraction and levels of viral proteins. HeLa cells were grown in chamber slides and infected with BPXV at an MOI of 5 for 1 h. Thiazovivin was applied at 4 hpi. At 15 hpi, BPXV (FITC) proteins were probed by immunofluorescence assay. Cell morphology and level of viral proteins of Thiazovivin-treated or untreated cells is shown (bi). The histogram shows the relative reduction in cell size (bii) and relative levels of BPXV proteins (biii) in Thiazovivin treated or untreated cells. The area (n = 50 cells) and the intensity of viral proteins (n = 50 cells) were quantified by ImageJ. The data are presented as mean with SD. Pair-wise statistical comparisons were performed using Student's t-test (***P < 0.001; **P < 0.001).

Article Snippet: African green monkey kidney (Vero) and HeLa cells were received from National Centre for Cell Science (NCCS), Pune, India.

Techniques: Inhibition, Phospho-proteomics, Activation Assay, Infection, Western Blot, Immunofluorescence

Journal: Molecules

Article Title: 2,5-Diketopiperazine Derivatives as Potential Anti-Influenza (H5N2) Agents: Synthesis, Biological Evaluation, and Molecular Docking Study

doi: 10.3390/molecules27134200

Figure Lengend Snippet: Summary of influenza virus (H5N2) propagation inhibition in embryonated chicken eggs of LS-C, LS-D, and 2,5-DKP derivatives detected by hemagglutination assay and the cytotoxicity activity (IC 50 ) against LLC-MK2.

Article Snippet: Cytotoxicity was evaluated against normal cell line Rhesus monkey kidney epithelial cells LLC-MK2 (Korean Cell Line Bank, KCLB) by using a MTT assay [ ].

Techniques: Virus, Inhibition, Hemagglutination Assay, Activity Assay